Impact of Chlorpyrifos on Cytopathological Indices in Mangrove Crab, Episesarma tetragonum (Fabricius)

Simple Summary The purpose of this study was to see how chlorpyrifos, an organophosphate insecticide commonly found in an aquatic environment, affected the cytotoxicity of the mangrove crab, Episesarma tetragonum. For 7 and 28 days, specimens were exposed to 0.0294 and 0.0588 ppm chlorpyrifos, respectively. The gills, hepatopancreas, and muscles were examined for cytopathological effects. The findings indicate that chlorpyrifos causes cytopathological changes such as decreased epithelial lifting, edema, necrosis, secondary lamellae fusion, haemorrhage, haemocyte disappearance, muscle atrophy, and necrosis. Therefore, cytopathologic observation serves as a promising biomarker for ecotoxicological studies. Chlorpyrifos is an organophosphate insecticide occurring in aquatic ecosystems. Due to exposure to xenobiotics, several harmful effects on aquatic organisms are noticed worldwide. Mangrove crabs are an ecologically important aquatic invertebrate species in food web interactions and in the mangrove ecosystem. Therefore, this study aimed to evaluate the cytotoxic effects of chlorpyrifos on the mangrove crab, Episesarma tetragonum. Crabs were exposed to 0.0294 and 0.0588 ppm of chlorpyrifos for 7 and 28 days. Cytopathologic effects on the gill, hepatopancreas, and muscle were investigated, and observations were compared with a control group. The results suggest that chlorpyrifos induces time- and concentration-dependent cytopathological alternations in the gill and exhibited epithelial lifting, oedema, necrosis, and a fusion of secondary lamellae and haemorrhage. The deceased hepatopancreas showed infiltration, a large lumen formation, and the disappearance of haemocytes, while the muscle tissue showed atrophy, necrosis, a wavy appearance, an accumulation of granular material between muscle fibres, and fragmentation in a mangrove crab. This study shows the great potential of cytopathological investigations, allows us to assess the sensitivity of various aquatic animal species to potentially dangerous compounds, and calculates safe concentrations with which to reduce pesticide use.

Automated summary

The aim of this study was to investigate the cytotoxic effects of the organophosphate insecticide chlorpyrifos on the mangrove crab. The results showed that chlorpyrifos caused cytopathological changes such as epithelial lifting, edema, necrosis, lamellae fusion, haemorrhage, haemocyte disappearance, muscle atrophy, and necrosis. Therefore, cytopathological observation is a promising biomarker for ecotoxicological studies.