Study of exopolysaccharide produced by Streptomyces rochie strain OF1 and its effect as ameliorative on osteoarthritis in rats via inhibiting TNF-α/COX2 pathway

BackgroundCarbohydrates are known as the main natural products of life activities.ResultsStreptomyces rochie strain OF1 isolated from a mangrove tree produced exopolysaccharide S5 (EPSS5) (14.2 gl(-1)) containing uronic acid 21.98% sulfate content of 11.65 mg/ml, and a viscosity of 1.35 mm(2)/s. while total hexose amine content was 24.72%. The high performance liquid chromatography (HPLC) analysis of mono sugars revealed that EPS was composed of manouronic acid, glucuronic acid, xylose, and fructose at a molar ratio of 1.0:0.5:1.0:2.0, respectively. It showed that the whole antioxidant activity was 92.06%. It showed antibacterial activity against Staphylococcus aureus, and E. coli, MRSA and Klebsiella pneumoniae. But, EPSS5 displayed low antifungal activity against Candida albicans. While no antifungal activity has been detected against Aspergillus niger. EPSS5 has antibiofilm action that is noticeable toward S. aureus with an inhibition ratio of biofilm up to 50%. Effect of EPS on serum levels of TNF-alpha and COX2 by 2 fold and 1.9 fold of EPS reduced serum levels of Tumor necrosis factor-alpha (TNF-alpha) by 38%, 12%, 49%, and Cyclooxygenase-2 (COX2) by 61%, 34%, and 62%, respectively. By affected of EPSS5 on arthritis in rats stimulated by carrageenan.ConclusionsAdministration of EPS ameliorated carrageen-induced elevation in inflammatory mediators; TNF-alpha/COX and suppressed the expressions of metalloproteinase 9 (MMP9) by 68%, 86%, and 75% correspondingly in comparison to the group of carrageenans. Then again, therapy involving a high dose only reduced MMP9 level by 57%, compared to free drug suggesting that EPSS5 is a good inhibitor of the MMP9, as it brought MMP9 back to normal levels via the signaling pathway.

Automated summary

In this study, a strain of Streptomyces rochie isolated from a mangrove tree produced an exopolysaccharide S5 (EPSS5) with high antioxidant and antibacterial activities, but low antifungal activity. EPSS5 exhibited therapeutic effects on carrageenan-induced arthritis in rats by suppressing the expression of inflammatory mediators and regulating the signaling pathway.