Elicitation for activation of the actinomycete genome's cryptic secondary metabolite gene clusters

This review summarizes the recent advances in the elicitation approaches used to activate the actinomycete genome's cryptic secondary metabolite gene clusters and shows the diversity of natural products obtained by various elicitation methods up to June 2022, such as co-cultivation of actinomycetes with actinomycetes, other non-actinomycete bacteria, fungi, cell-derived components, and/or algae. Chemical elicitation and molecular elicitation as transcription factor decoys, engineering regulatory genes, the promoter replacement strategy, global regulatory genes, and reporter-guided mutant selection were also reported. For researchers interested in this field, this review serves as a valuable resource for the latest studies and references.

Automated summary

This review summarizes recent advances in the elicitation approaches used to activate cryptic secondary metabolite gene clusters in the actinomycete genome, highlighting the diversity of natural products obtained through various elicitation methods until June 2022, including co-cultivation with actinomycetes, non-actinomycete bacteria, fungi, cell-derived components, and/or algae. Chemical and molecular elicitation strategies such as transcription factor decoys, engineering regulatory genes, promoter replacement, global regulatory genes, and reporter-guided mutant selection were also discussed. This review serves as a valuable resource for researchers interested in this field, providing the latest studies and references.