Tutorial: multiphoton microscopy to advance neuroscience research

Multiphoton microscopy (MPM) employs ultrafast infrared lasers for high-resolution deep three-dimensional imaging of live biological samples. The goal of this tutorial is to provide a practical guide to MPM imaging for novice microscopy developers and life-science users. Principles of MPM, microscope setup, and labeling strategies are discussed. Use of MPM to achieve unprecedented imaging depth of whole mounted explants and intravital imaging via implantable glass windows of the mammalian nervous system is demonstrated.

Automated summary

Multiphoton microscopy (MPM) is a technique that uses ultrafast infrared lasers to achieve high-resolution three-dimensional imaging of live biological samples. This tutorial serves as a practical guide for novice microscopy developers and life-science users, covering the principles of MPM, microscope setup, and labeling strategies. The use of MPM to achieve unprecedented imaging depth of whole mounted explants and intravital imaging via implantable glass windows of the mammalian nervous system is demonstrated.