Thermodynamic and voltammetric study on carnosine and ferrocenyl-carnosine

A potentiometric study on the interactions of l-carnosine (CAR) (2-[(3-aminopropanoyl)amino]-3-(1H-imidazol-5-yl)propanoic acid) with two toxic metal cations, Hg2+ and Cd2+, is reported here. The elucidation of the metal (M2+)-CAR interactions in aqueous solution highlighted the speciation model for each system, the dependence of the formation constants of the complex species on ionic strength (0.15 <= I/mol L-1 <= 1) and temperature (288.15 <= T/K <= 310.15) and changes in enthalpy and entropy. The sequestering ability of CAR towards the two metal ions was quantified and compared with that with Pb2+, previously determined. Considering the complexing ability of CAR and its unclear electrochemical properties, a more electroactive derivative, the ferrocenyl-carnosine (FcCAR), was synthesized and its complexing ability was evaluated by UV-vis spectroscopy. FcCAR electrochemical properties were investigated by Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV) on Screen-Printed Electrodes (SPEs), to evaluate its sensing properties. Electrochemical responses in the presence of Hg2+ and Pb2+ have been shown to be promising for the electrochemical detection of these metal cations in aqueous environment.

Automated summary

A potentiometric study was conducted on the interactions between l-carnosine (CAR) and the toxic metal cations Hg2+ and Cd2+. The study determined the formation constants of the metal (M2+)-CAR complex species in aqueous solution and investigated the influence of ionic strength and temperature on these constants. The sequestering ability of CAR towards Hg2+ and Cd2+ was compared with that towards Pb2+. Additionally, a more electroactive derivative called ferrocenyl-carnosine (FcCAR) was synthesized and evaluated for its complexing ability and sensing properties towards Hg2+ and Pb2+ using UV-vis spectroscopy and electrochemical techniques.