Denaturation bubble-mediated two-stage isothermal nucleic acid amplification in a single closed tube

Here, we established a two-stage isothermal amplification method comprising strand exchange amplification and hyperbranched rolling circle amplification, which only employed one DNA polymerase and was performed in a single closed tube at a constant temperature, providing a promising signal amplification strategy for accurate and rapid pathogen detection in the clinic.

Automated summary

Here, we developed a two-stage isothermal amplification method using strand exchange amplification and hyperbranched rolling circle amplification. This method only requires one DNA polymerase and can be performed in a single closed tube at a constant temperature, offering a promising signal amplification strategy for accurate and rapid pathogen detection in clinical settings.