Journal
PROSTHESIS
Volume 5, Issue 1, Pages 1-12Publisher
MDPI
DOI: 10.3390/prosthesis5010001
Keywords
diagnosis; molecular biology; PCR; next-generation sequencing; metagenomics; cost; implementation; microbiology
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Despite recent advances, microbiological diagnosis of prosthetic joint infections remains challenging. Molecular biology techniques, including multiplex PCR assays and metagenomics, have shown potential but are still experimental. A few studies have evaluated the use of these methods in clinical routine and proposed protocols. However, their interpretation requires a multidisciplinary team with experience.
Despite recent advances during the last few years, microbiological diagnosis of prosthetic joint infections remains a challenge. Molecular biology techniques have been developed to try to overcome this problem, and recently, many of them have become available for many laboratories. Some of them, especially commercial multiplex PCR-based assays and universal 16S rDNA homemade PCR assays, are now available in many laboratories. Moreover, new technologies have appeared, especially metagenomics and next-generation sequencing. These techniques have demonstrated their potential in many studies but appear to be experimental at present. A few studies have evaluated the possible use of these methods in the clinical routine, and a review of the critical aspects for the selection of a molecular method (accuracy, complexity, cost) was performed. Finally, a proposal for a protocol that includes molecular biology techniques was made according to the literature published in this field. In conclusion, molecular biology techniques are ready to be used in the clinical routine of a microbiology laboratory, but their use must be carried out in accordance with the many special characteristics of each laboratory. In all cases, the interpretation of the results must be conducted by a multidisciplinary team with experience in the management of these patients.
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