Journal
BIOPHYSICS AND PHYSICOBIOLOGY
Volume 20, Issue 1, Pages -Publisher
BIOPHYSICAL SOC JAPAN
DOI: 10.2142/biophysico.bppb-v20.0006
Keywords
HS-AFM; piezo hysteresis; computational method
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The high-speed atomic force microscopy (HS-AFM) is a unique and prominent method to observe structural dynamics of biomolecules at single molecule level at near-physiological condition. In this study, a computational method was developed to detect and remove the parachuting artifact in HS-AFM images using the two-way scanning data.
The high-speed atomic force microscopy (HS-AFM) is a unique and prominent method to observe structural dynamics of biomolecules at single molecule level at near-physiological condition. To achieve high temporal resolution, the probe tip scans the stage at high speed which can cause the so-called parachuting artifact in the HS-AFM images. Here, we develop a computational method to detect and remove the parachuting artifact in HS-AFM images using the two-way scanning data. To merge the two-way scanning images, we employed a method to infer the piezo hysteresis effect and to align the forward-and backward-scanning images. We then tested our method for HS-AFM videos of actin filaments, molecular chaperone, and duplex DNA. Together, our method can remove the parachuting artifact from the raw HS-AFM video containing two-way scanning data and make the processed video free from the parachuting artifact. The method is general and fast so that it can easily be applied to any HS-AFM videos with two-way scanning data.
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