EFFECT OF APPLICATION OF GROWTH ELICITORS AND INSILICO ANALYSIS OF REGULATORY PROTEINS IN SWEET PEA (PISUM SATIVUM L.) AGAINST DROUGHT STRESS

Drought stress has an impact on the growth and yield of sweet pea (Pisum sativum). Drought stress has a negative impact on morphological and physiological characteristics including as height, shoot and root length, leaf area, fresh and dry weight, anthocyanin, chlorophyll a and b, soluble protein, and H2O2. The salicylic acid (SA) and ascorbic acid (AA) are used to modulate plant response against dry conditions and provide protection against oxidative damage. To overcome this loss SA and AA (0.5, 1 and 1.5 mM) were used by foliar application. Lab work was performed to illustrate the harmful effects of drought stress and beneficial role of SA and AA on P. sativum. These effects of drought stress were analysed by different computational analyses. The drought stress is very much effective at proteomic level and some proteins like PIP2-2, DREB2A and 14-3-3 like proteins are activated which prevent the plant from such dangerous effects. The activation of these proteins is up -regulated by SA application. So in dehydrated conditions it would be better choice to use SA and AA to compensate the shortage of water for plant. Because these compounds provide resistance against drought stress and restore the damages caused by drought stress and enable the plant to survive in such harmful conditions.

Automated summary

Drought stress negatively affects the growth and physiological characteristics of sweet pea. Salicylic acid and ascorbic acid are used to enhance plant response and protect against oxidative damage. Lab work demonstrates the harmful impact of drought stress and the beneficial role of SA and AA on sweet pea.