Journal
HORTICULTURAL PLANT JOURNAL
Volume 9, Issue 2, Pages 335-344Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.hpj.2022.06.001
Keywords
Antioxidant activity; Flavonoid; Herbaceous peony petal; Oxidative stress; Solid-phase extraction
Categories
Ask authors/readers for more resources
This study aimed to investigate the flavonoid profiles and antioxidant activity of Paeonia lactiflora petal flavonoid extract (PPF). The UHPLC-ESI-Q-Exactive HF MS/MS method was established for characterization, and 21 predominant flavonoid compounds were tentatively identified in PPF. The results suggested that herbaceous peony flavonoids can be used as a natural bioactive agent to prevent oxidative stress.
Flavonoids have attracted considerable attention due to their health benefits. This study aimed to investigate the flavonoid profiles and antioxidant activity of Paeonia lactiflora petal flavonoid extract (PPF). The UHPLC-ESI-Q-Exactive HF MS/MS method was established for characterization, and 21 predominant flavonoid compounds were tentatively identified in PPF. Among them, isoscutellarein-7-(60-acety-lallosyl-(1->2)-glucoside) and scutellarin methylester were discovered in PPF for the first time. Pretreatment with PPF significantly reduced H2O2-induced cell damage, ROS accumulation, and malondialdehyde content and increased the activity of SOD, CAT, and GSH-Px in buffalo rat liver 3A (BRL3A) cells. Moreover, the expression of nuclear Factor E2-related factor (Nrf2) was upregulated by PPF, whose expression trend was consistent with that of heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), and NAD(P)H quinone oxidoreductase-1 (NQO1). These findings suggested that herbaceous peony flavonoids can be used as a natural bioactive agent to prevent oxidative stress.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available