4.2 Article

NanoB2 to monitor interactions of ligands with membrane proteins by combining nanobodies and NanoBRET

Related references

Note: Only part of the references are listed.
Article Biochemical Research Methods

Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels

Jonathan B. Grimm et al.

Summary: This Perspective provides an overview of the benefits and peculiarities of commonly used organic dyes in biological imaging from a chemist's perspective, also offering frank advice on navigating the challenges that come with using these dyes for biologists adopting new technologies.

NATURE METHODS (2022)

Article Biochemistry & Molecular Biology

INDI-integrated nanobody database for immunoinformatics

Piotr Deszynski et al.

Summary: Nanobodies, a subclass of antibodies found in camelids, are composed of a single polypeptide chain and offer therapeutic advantages. The Integrated Database of Nanobodies for Immunoinformatics (INDI) collates nanobody information from various public sources to facilitate the development of novel computational protocols specific to nanobodies.

NUCLEIC ACIDS RESEARCH (2022)

Review Biochemistry & Molecular Biology

Accelerating GPCR Drug Discovery With Conformation-Stabilizing VHHs

Toon Laeremans et al.

Summary: GPCRs are potential drug targets but discovering drugs targeting them is challenging. Stabilizing and studying GPCR structures is crucial, and camelid-derived immunoglobulin single variable domains (VHHs) can be powerful tools.

FRONTIERS IN MOLECULAR BIOSCIENCES (2022)

Article Immunology

Fluorescently tagged nanobodies and NanoBRET to study ligand-binding and agonist-induced conformational changes of full-length EGFR expressed in living cells

Dehan Comez et al.

Summary: In this study, NanoBRET technique was used to compare the binding characteristics of two distinct EGFR targeting nanobodies to full length EGFR. The results showed that these nanobodies had different functions, with one inhibiting EGF binding to EGFR and the other acting as a conformational sensor for agonist bound EGFR.

FRONTIERS IN IMMUNOLOGY (2022)

Review Chemistry, Medicinal

New small molecule fluorescent probes for G protein-coupled receptors: valuable tools for drug discovery

Joshua W. Conner et al.

Summary: This review addresses the key challenges, synthesis approaches, and structure-activity relationships in recent fluorescent small molecule studies for GPCRs, and discusses the advantages of using high-resolution GPCR structures to inform conjugation strategies.

FUTURE MEDICINAL CHEMISTRY (2021)

Article Cell Biology

Differential Involvement of ACKR3 C-Tail in β-Arrestin Recruitment, Trafficking and Internalization

Aurelien Zarca et al.

Summary: The study revealed that ACKR3 recruits beta-arrestins and internalizes to the cell membrane upon CXCL12 stimulation, with GRK2 and 3 playing key roles in beta-arrestin recruitment and receptor internalization.

CELLS (2021)

Article Multidisciplinary Sciences

Nanobody cocktails potently neutralize SARS-CoV-2 D614G N501Y variant and protect mice

Phillip Pymm et al.

Summary: Neutralizing antibodies, particularly nanobodies, play a crucial role in immunity against SARS-CoV-2 and in the development of therapeutics for COVID-19. Researchers have identified high-affinity nanobodies that effectively disrupt the interaction between the virus and the human host cell receptor ACE2, offering promising prophylactic potential against SARS-CoV-2. Studies have shown that nanobody-Fc fusions can block viral engagement with host cells and significantly reduce viral loads in infected mice, suggesting their use as preventive agents against COVID-19.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Biology

Biased action of the CXCR4-targeting drug plerixafor is essential for its superior hematopoietic stem cell mobilization

Astrid S. Jorgensen et al.

Summary: Plerixafor exhibits biased action by reversing the CXCL12 gradient across the bone marrow endothelium and showing superior therapeutic effect in HSC mobilization. In contrast, AMD11070, despite having superior antagonism, does not perform as effectively in HSC mobilization in vivo.

COMMUNICATIONS BIOLOGY (2021)

Article Multidisciplinary Sciences

Selective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies

Timo W. M. De Groof et al.

Summary: The study demonstrates that different nanobodies can recognize and block distinct conformational states of GPCR US28, achieving selective targeting of ligand-dependent versus independent signaling and discerning functional consequences.

NATURE COMMUNICATIONS (2021)

Article Multidisciplinary Sciences

A potent SARS-CoV-2 neutralising nanobody shows therapeutic efficacy in the Syrian golden hamster model of COVID-19

Jiandong Huo et al.

Summary: Nanobodies engineered as homotrimers show promising potential in neutralizing SARS-CoV-2 viral strains for COVID-19 treatment, especially when administered through respiratory routes. The study characterizes four nanobodies with high affinity for the receptor binding domain of the spike protein, demonstrating efficacy in inhibiting disease progression in animal models.

NATURE COMMUNICATIONS (2021)

Article Medicine, General & Internal

D-dopachrome tautomerase contributes to lung epithelial repair via atypical chemokine receptor 3-dependent Akt signaling

Shanshan Song et al.

Summary: DDT contributes to alveolar epithelial repair via ACKR3 by promoting proliferation and preventing apoptosis of lung epithelial cells, stimulating growth of primary alveolar epithelial cells, and enhancing activation of the PI3K-Akt pathway.

EBIOMEDICINE (2021)

Review Oncology

The Therapeutic Potential of Nanobodies

Ivana Jovcevska et al.

BIODRUGS (2020)

Article Chemistry, Multidisciplinary

Improved HaloTag Ligand Enables BRET Imaging With NanoLuc

Ovia Margaret Thirukkumaran et al.

FRONTIERS IN CHEMISTRY (2020)

Article Chemistry, Analytical

Development of a Novel SPR Assay to Study CXCR4-Ligand Interactions

Arnaud Boonen et al.

BIOSENSORS-BASEL (2020)

Article Biochemistry & Molecular Biology

Monitoring Allosteric Interactions with CXCR4 Using NanoBiT Conjugated Nanobodies

Mark Soave et al.

CELL CHEMICAL BIOLOGY (2020)

Review Cell Biology

Nanobodies: New avenues for imaging, stabilizing and modulating GPCRs

Timo W. M. De Groof et al.

MOLECULAR AND CELLULAR ENDOCRINOLOGY (2019)

Review Cell Biology

Nanobodies detecting and modulating GPCRs outside in and inside out

Raimond Heukers et al.

CURRENT OPINION IN CELL BIOLOGY (2019)

Article Biochemistry & Molecular Biology

Engineering of Nanobodies Recognizing the Human Chemokine Receptor CCR7

Barbara D. Jakobs et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)

Review Biochemistry & Molecular Biology

Drug-Target Association Kinetics in Drug Discovery

Adriaan P. IJzerman et al.

TRENDS IN BIOCHEMICAL SCIENCES (2019)

Article Medicine, Research & Experimental

Nanobody-Targeted Photodynamic Therapy Selectively Kills Viral GPCR-Expressing Glioblastoma Cells

Timo W. M. De Groof et al.

MOLECULAR PHARMACEUTICS (2019)

Review Biotechnology & Applied Microbiology

NanoBRET: The Bright Future of Proximity-Based Assays

Natasha C. Dale et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2019)

Review Pharmacology & Pharmacy

Intracellular Receptor Modulation: Novel Approach to Target GPCRs

Natalia V. Ortiz Zacarias et al.

TRENDS IN PHARMACOLOGICAL SCIENCES (2018)

Article Pharmacology & Pharmacy

CXCR4-targeting nanobodies differentially inhibit CXCR4 function and HIV entry

Anneleen Van Hout et al.

BIOCHEMICAL PHARMACOLOGY (2018)

Review Chemistry, Medicinal

GPCR Allosteric Modulators: Mechanistic Advantages and Therapeutic Applications

Eric A. Wold et al.

CURRENT TOPICS IN MEDICINAL CHEMISTRY (2018)

Editorial Material Biochemical Research Methods

sdAb-DB: The Single Domain Antibody Database

Emily E. Wilton et al.

ACS SYNTHETIC BIOLOGY (2018)

Review Biochemistry & Molecular Biology

Drug-Target Kinetics in Drug Discovery

Peter J. Tonge

ACS CHEMICAL NEUROSCIENCE (2018)

Article Biotechnology & Applied Microbiology

Trends in GPCR drug discovery: new agents, targets and indications

Alexander S. Hauser et al.

NATURE REVIEWS DRUG DISCOVERY (2017)

Article Biochemistry & Molecular Biology

EGFR Ligands Differentially Stabilize Receptor Dimers to Specify Signaling Kinetics

Daniel M. Freed et al.

Review Pharmacology & Pharmacy

Kinetics for Drug Discovery: an industry-driven effort to target drug residence time

Doris A. Schuetz et al.

DRUG DISCOVERY TODAY (2017)

Review Biotechnology & Applied Microbiology

Nanobody-based cancer therapy of solid tumors

Marta Kijanka et al.

NANOMEDICINE (2015)

Article Biochemical Research Methods

Application of BRET to monitor ligand binding to GPCRs

Leigh A. Stoddart et al.

NATURE METHODS (2015)

Review Neurosciences

Probing the pharmacology of G protein-coupled receptors with fluorescent ligands

Leigh A. Stoddart et al.

NEUROPHARMACOLOGY (2015)

Article Biochemical Research Methods

A robust pipeline for rapid production of versatile nanobody repertoires

Peter C. Fridy et al.

NATURE METHODS (2014)

Review Endocrinology & Metabolism

Allosteric and biased G protein-coupled receptor signaling regulation: potentials for new therapeutics

Etienne Khoury et al.

FRONTIERS IN ENDOCRINOLOGY (2014)

Article Pharmacology & Pharmacy

Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes

A. O. Watts et al.

BRITISH JOURNAL OF PHARMACOLOGY (2013)

Article Biochemistry & Molecular Biology

Structural Evaluation of EGFR Inhibition Mechanisms for Nanobodies/VHH Domains

Karl R. Schmitz et al.

STRUCTURE (2013)

Article Biochemistry & Molecular Biology

Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate

Mary P. Hall et al.

ACS CHEMICAL BIOLOGY (2012)

Article Biochemical Research Methods

A simple, versatile method for GFP-based super-resolution microscopy via nanobodies

Jonas Ries et al.

NATURE METHODS (2012)

Article Multidisciplinary Sciences

Preparation of unnatural N-to-N and C-to-C protein fusions

Martin D. Witte et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

Article Multidisciplinary Sciences

CXCR4 nanobodies (VHH-based single variable domains) potently inhibit chemotaxis and HIV-1 replication and mobilize stem cells

Sven Jahnichen et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010)

Article Multidisciplinary Sciences

Human cytomegalovirus-encoded chemokine receptor US28 promotes tumorigenesis

David Maussang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)

Article Multidisciplinary Sciences

Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies

E De Genst et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)

Article Biochemistry & Molecular Biology

Molecular mechanism of AMD3100 antagonism in the CXCR4 receptor - Transfer of binding site to the CXCR3 receptor

MM Rosenkilde et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2004)

Review Biochemistry & Molecular Biology

An open-and-shut case? Recent insights into the activation of EGF/ErbB receptors

AW Burgess et al.

MOLECULAR CELL (2003)