A novel QTL associated with tolerance to cold-induced seed cracking in the soybean cultivar Toyomizuki

Low temperatures after flowering cause seed cracking (SC) in soybean. Previously, we reported that proanthocyanidin accumulation on the dorsal side of the seed coat, controlled by the I locus, may lead to cracked seeds; and that homozygous IcIc alleles at the I locus confer SC tolerance in the line Toiku 248. To discover new genes related to SC tolerance, we evaluated the physical and genetic mechanisms of SC tolerance in the cultivar Toyomizuki (genotype II). Histological and texture analyses of the seed coat revealed that the ability to maintain hardness and flexibility under low temperature, regardless of proanthocyanidin accumulation in the dorsal seed coat, contributes to SC tolerance in Toyomizuki. This indicated that the SC tolerance mechanism differed between Toyomizuki and Toiku 248. A quantitative trait loci (QTL) analysis of recombinant inbred lines revealed a new, stable QTL related to SC tolerance. The relationship between this new QTL, designated as qCS8-2, and SC tolerance was confirmed in residual heterozygous lines. The distance between qCS8-2 and the previously identified QTL qCS8-1, which is likely the Ic allele, was estimated to be 2-3 Mb, so it will be possible to pyramid these regions to develop new cultivars with increased SC tolerance.

Automated summary

Low temperatures after flowering can cause seed cracking in soybean. Previous studies have shown that proanthocyanidin accumulation on the dorsal side of the seed coat, controlled by the I locus, may lead to cracked seeds, but the cultivar Toyomizuki showed a different mechanism for seed cracking tolerance. Through histological and texture analyses, it was found that the ability to maintain hardness and flexibility under low temperature, regardless of proanthocyanidin accumulation, contributed to the seed cracking tolerance in Toyomizuki. A new stable QTL, designated as qCS8-2, was identified and confirmed to be related to seed cracking tolerance, which could be used for developing new cultivars with increased tolerance.