An ultrasensitive mix-and-detect kind of fluorescent biosensor for malaoxon detection using the AChE-ATCh-Ag-GO system

Malaoxon, a highly toxic metabolite of malathion, can lead to severe harm or death if ingested. This study introduces a rapid and innovative fluorescent biosensor that relies on acetylcholinesterase (AChE) inhibition for detecting malaoxon using Ag-GO nanohybrid. The synthesized nanomaterials (GO, Ag-GO) were evaluated with multiple characterization methods to confirm their elemental composition, morphology, and crystalline structure. The fabricated biosensor works by utilizing AChE to catalyze the substrate acetylthiocholine (ATCh), which generates positively charged thiocholine (TCh) and triggers citrate-coated AgNP aggregation on the GO sheet, leading to an increase in fluorescence emission at 423 nm. However, the presence of malaoxon inhibits the AChE action and reduces the production of TCh, resulting in a decrease in fluorescence emission intensity. This mechanism allows the biosensor to detect a wide range of malaoxon concentrations with excellent linearity and low LOD and LOQ values of 0.001 pM to 1000 pM, 0.9 fM, and 3 fM, respectively. The biosensor also demonstrated superior inhibitory efficacy towards malaoxon compared to other OP pesticides, indicating its resistance to external influences. In practical sample testing, the biosensor displayed recoveries of over 98% with extremely low RSD% values. Based on the results obtained from the study, it can be concluded that the developed biosensor has the potential to be used in various real-world applications for detecting malaoxon in food, and water samples, with high sensitivity, accuracy, and reliability.

Automated summary

This study presents a rapid and innovative fluorescent biosensor based on acetylcholinesterase (AChE) inhibition for detecting malaoxon. The biosensor utilizes AChE to catalyze the substrate ATCh, triggering citrate-coated AgNP aggregation and resulting in increased fluorescence emission. However, the presence of malaoxon inhibits AChE action, leading to decreased fluorescence emission. The biosensor demonstrates excellent linearity and low detection limits, showing potential for real-world applications in detecting malaoxon with high sensitivity and accuracy.