Journal
ADVANCES IN NANO RESEARCH
Volume 14, Issue 4, Pages 391-397Publisher
TECHNO-PRESS
DOI: 10.12989/anr.2023.14.4.391
Keywords
anti-bacterial; anti-biofilm; characterization; nanoparticles; phytosynthesis
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This manuscript describes the one-step eco-friendly green fabrication of silver nanoparticles (AgNPs) through in-situ bio-reduction of silver nitrate using Syzygium aromaticum leaf extract. The UV-vis spectroscopy showed a characteristic SPR peak around 442 nm. FTIR spectroscopy confirmed the presence of bioactive phyto-molecules on the AgNPs. TEM images revealed oval and spherical particles with a mean diameter of 12.6 nm. XRD analysis confirmed the crystalline structure of the AgNPs. The phytosynthesized AgNPs exhibited broad-spectrum antimicrobial activity against two foodborne pathogenic bacteria, Listeria monocytogenes and Staphylococcus aureus, and effectively inhibited biofilm formation.
This manuscript describes the one-step eco-friendly green fabrication of silver nanoparticles (AgNPs) through the in-situ bio-reduction of an aqueous solution of silver nitrate using Syzygium aromaticum leaf extract. UV-vis spectroscopy shows a characteristic SPR peak around 442 nm. FTIR spectroscopy showed that the AgNPs were capped with bioactive phyto-molecules. TEM images revealed oval and spherical particles with a mean diameter of similar to 12.6 nm. XRD analysis revealed crystalline and face-cantered cubic AgNPs. The phytosynthesized AgNPs showed broad-spectrum anti-microbial activity against two foodborne pathogenic bacteria, Listeria monocytogenes and Staphylococcus aureus. The AgNPs showed a prominent ability to inhibit biofilms formed by L. monocytogenes and S. aureus in laboratory conditions through a crystal violet assay. The results suggest that the AgNPs could be a novel nanotool to develop effective antimicrobial and anti-biofilm agents in food preservation.
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