Journal
CURRENT OPINION IN VIROLOGY
Volume 18, Issue -, Pages 57-63Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.coviro.2016.02.011
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Funding
- National Science Foundation [0836656, 0735297]
- PEW Latin American Fellowship Program
- Conselho Nacional de Desenvolvimento Cientifico (CNPq), Brazil
- Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ), Brazil
- NIH [R01 GM054076]
- Direct For Computer & Info Scie & Enginr
- Division of Computing and Communication Foundations [1217867, 0735297] Funding Source: National Science Foundation
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [0836656] Funding Source: National Science Foundation
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The direct electron detector has revolutionized electron cryo-microscopy (CryoEM). Icosahedral virus structures are routinely produced at 4 A resolution or better and the approach has largely displaced virus crystallography, as it requires less material, less purity and often produces a structure more rapidly. Largely ignored in this new era of CryoEM is the dynamic information in the data sets that was not available in X-ray structures. Here we review an approach that captures the dynamic character of viruses displayed in the CryoEM ensemble of particles at the moment of freezing. We illustrate the approach with a simple model, briefly describe the details and provide a practical application to virus particle maturation.
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