A LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP)-BASED POINT-OF-CARE SYSTEM FOR RAPID ON-SITE CLINICAL DETECTION OF SARS-COV-2 VIRUSES

In the ongoing COVID-19 pandemic, sensitive and rapid on-site detection of the SARS-CoV-2 coronavirus has been one of crucial objectives. A point-of-care (PoC) device called PATHPOD for quick, on-site detection of SARS-CoV-2 employing a real-time reverse-transcription loop-mediated isothermal amplification (RT-rLAMP) reaction on a polymer cartridge. The PATHPOD consists of a standalone device (weighing under 1.2 kg) and a cartridge, and can identify 10 distinct samples and 2 controls in less than 50 minutes. The PATHPOD PoC system is fabricated and clinically validated for the first time in this work

Automated summary

Sensitive and rapid on-site detection of SARS-CoV-2 using the PATHPOD device, which employs real-time reverse-transcription loop-mediated isothermal amplification (RT-rLAMP) reaction on a polymer cartridge, has been achieved. The PATHPOD PoC system, consisting of a standalone device and a cartridge, can identify 10 distinct samples and 2 controls in less than 50 minutes. This work presents the fabrication and clinical validation of the PATHPOD PoC system for the first time.