Journal
CELL REPORTS
Volume 15, Issue 4, Pages 748-760Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2016.03.060
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Funding
- NIH-NINDS [R37NS042925, NS-R0152738, F31NS077504]
- UK Multiple Sclerosis Society
- NIH-NIMH [R01MH090948]
- Medical Research Council [MC_PC_12009] Funding Source: researchfish
- Rosetrees Trust [M144] Funding Source: researchfish
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Oligodendrocytes derive from progenitors (OPCs) through the interplay of epigenomic and transcriptional events. By integrating high-resolution methylomics, RNA-sequencing, and multiple transgenic lines, this study defines the role of DNMT1 in developmental myelination. We detected hypermethylation of genes related to cell cycle and neurogenesis during differentiation of OPCs, yet genetic ablation of Dnmt1 resulted in inefficient OPC expansion and severe hypomyelination associated with ataxia and tremors in mice. This phenotype was not caused by lineage switch or massive apoptosis but was characterized by a profound defect of differentiation associated with changes in exon-skipping and intron-retention splicing events and by the activation of an endoplasmic reticulum stress response. Therefore, loss of Dnmt1 in OPCs is not sufficient to induce a lineage switch but acts as an important determinant of the coordination between RNA splicing and protein synthesis necessary for myelin formation.
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