Journal
CELL REPORTS
Volume 14, Issue 11, Pages 2637-2652Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2016.02.046
Keywords
-
Categories
Funding
- NIH [GM48661, F30NS092227]
- UPenn NGG Hearst Fellowship
- Institut Curie
- CNRS
- INSERM
- ANR [ANR-12-BSV2-0007, INCA_6517]
- IDEX Idex PSL [ANR-10-LBX-0038, ANR-10-IDEX-0001-02 PSL]
- [R25 GM103792-01]
- Agence Nationale de la Recherche (ANR) [ANR-12-BSV2-0007] Funding Source: Agence Nationale de la Recherche (ANR)
Ask authors/readers for more resources
Motor-cargo recruitment to microtubules is often the rate-limiting step of intracellular transport, and defects in this recruitment can cause neurodegenerative disease. Here, we use in vitro reconstitution assays with single-molecule resolution, live-cell transport assays in primary neurons, computational image analysis, and computer simulations to investigate the factors regulating retrograde transport initiation in the distal axon. We find that phosphorylation of the cytoskeletal-organelle linker protein CLIP-170 and post-translational modifications of the microtubule track combine to precisely control the initiation of retrograde transport. Computer simulations of organelle dynamics in the distal axon indicate that while CLIP-170 primarily regulates the time to microtubule encounter, the tyrosination state of the microtubule lattice regulates the likelihood of binding. These mechanisms interact to control transport initiation in the axon in a manner sensitive to the specialized cytoskeletal architecture of the neuron.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available