Journal
CELL REPORTS
Volume 15, Issue 12, Pages 2771-2783Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2016.05.059
Keywords
-
Categories
Funding
- National Institute on Drug Abuse (NIDA) [F31DA036425]
- NIH National Institute of Allergy and Infectious Diseases (NIAID) [R01AI074420]
- National Institute of General Medical Sciences [GM113885]
- NIDA Avant Garde [DA028866]
- Burroughs Wellcome Investigators in the Pathogenesis of Infectious Disease Fund
- NIH NIAID [R01AI093998]
Ask authors/readers for more resources
HIV-1 infection is enhanced by adhesive structures that form between infected and uninfected T cells called virological synapses (VSs). This mode of transmission results in the frequent co-transmission of multiple copies of HIV-1 across the VS, which can reduce sensitivity to antiretroviral drugs. Studying HIV-1 infection of humanized mice, we measured the frequency of co-transmission and the spatiotemporal organization of infected cells as indicators of cell-to-cell transmission in vivo. When inoculating mice with cells co-infected with two viral genotypes, we observed high levels of co-transmission to target cells. Additionally, micro-anatomical clustering of viral genotypes within lymphoid tissue indicates that viral spread is driven by local processes and not a diffuse viral cloud. Intravital splenic imaging reveals that anchored HIV-infected cells induce arrest of interacting, uninfected CD4(+) T cells to form Envdependent cell-cell conjugates. These findings suggest that HIV-1 spread between immune cells can be anatomically localized into infectious clusters.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available