Journal
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 30, Issue 30, Pages 75281-75299Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-023-27662-2
Keywords
Locomotor assay; Spontaneous tail movement; Danio rerio; Behavior profiling; Developmental toxicity testing; Alternative test method
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In order to determine neurotoxicity, new methods are being validated, including both in vitro and in vivo tests. One of the alternative test models attracting attention is the zebrafish embryo, specifically for assessing behavioral endpoints related to neurotoxicity during early development stages. This study investigated the sensitivity of the coiling assay, a method to assess random movement development, to different neurotoxicants with various modes of action.
In the aim to determine neurotoxicity, new methods are being validated, including tests and test batteries comprising in vitro and in vivo approaches. Alternative test models such as the zebrafish (Danio rerio) embryo have received increasing attention, with minor modifications of the fish embryo toxicity test (FET; OECD TG 236) as a tool to assess behavioral endpoints related to neurotoxicity during early developmental stages. The spontaneous tail movement assay, also known as coiling assay, assesses the development of random movement into complex behavioral patterns and has proven sensitive to acetylcholine esterase inhibitors at sublethal concentrations. The present study explored the sensitivity of the assay to neurotoxicants with other modes of action (MoAs). Here, five compounds with diverse MoAs were tested at sublethal concentrations: acrylamide, carbaryl, hexachlorophene, ibuprofen, and rotenone. While carbaryl, hexachlorophene, and rotenone consistently induced severe behavioral alterations by similar to 30 h post fertilization (hpf), acrylamide and ibuprofen expressed time- and/or concentration-dependent effects. At 37-38 hpf, additional observations revealed behavioral changes during dark phases with a strict concentration-dependency. The study documented the applicability of the coiling assay to MoA-dependent behavioral alterations at sublethal concentrations, underlining its potential as a component of a neurotoxicity test battery.
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