An activatable nanoprobe based on nanocomposites of visible-light-excitable europium(iii) complex-anchored MnO2 nanosheets for bimodal time-gated luminescence and magnetic resonance imaging of tumor cells

Bimodal imaging probes that combine magnetic resonance imaging (MRI) and photoluminescence imaging are quite appealing since they can supply both anatomical and molecular information to effectively ameliorate the accuracy of detection. In this study, an activatable nanoprobe, [Eu(BTD)(3)(DPBT)]@MnO2, for bimodal time-gated luminescence imaging (TGLI) and MRI has been constructed by anchoring visible-light-excitable Eu3+ complexes on lamellar MnO2 nanosheets. Due to the luminescence quenching effect and non-magnetic resonance (MR) activity of MnO2 nanosheets, the developed nanoprobe presents quite weak TGL and MR signals. After exposure to H2O2 or GSH, accompanied by the transformation from MnO2 to Mn2+, the nanoprobe exhibits rapid, sensitive, and selective turn-on responses towards GSH and H2O2 in TGL and MR detection modes. Furthermore, the nanoprobe displays high stability, low cytotoxicity, good biocompatibility and water dispersion. Given the high contents of GSH and H2O2 in cancer cells, the nanoprobe was used for the identification of cancer cells by TGLI of intracellular GSH and H2O2, as well as for the tracing of tumor cells in tumor-bearing mice by tumor-targeting in vivo MRI and TGLI of tumor tissues. The research outcomes proved the potential of [Eu(BTD)(3)(DPBT)]@MnO2 as a useful nanoprobe for the tracing and accurate detection of cancer cells in vitro and in vivo via bimodal TGLI and MRI.

Automated summary

In this study, an activatable nanoprobe for bimodal TGLI and MRI has been developed, which shows rapid, sensitive, and selective turn-on responses towards GSH and H2O2. The nanoprobe can be used for the identification and tracing of cancer cells in vitro and in vivo.