Journal
CELL REPORTS
Volume 15, Issue 9, Pages 1986-1999Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2016.04.078
Keywords
-
Categories
Funding
- HIVRAD [P01 AI104722]
- CHAVI-ID [AI100663, R01AI073148, AI098602]
- International AIDS Initiative (IAVI)
- Bill AMP
- Melinda Gates Foundation
- Ministry of Foreign Affairs of Denmark, Irish Aid
- Ministry of Finance of Japan
- Ministry of Foreign Affairs of the Netherlands
- Norwegian Agency for Development Cooperation (NORAD)
- United Kingdom Department for International Development (DFID)
- U.S. Agency for International Development (USAID)
- Bill AMP
- Melinda Gates Foundation Collaboration for AIDS Vaccine Discovery
Ask authors/readers for more resources
A major step toward an HIV-1 vaccine is an immunogen capable of inducing neutralizing antibodies. Envelope glycoprotein (Env) mimetics, such as the NFL and SOSIP designs, generate native-like, well-ordered trimers and elicit tier 2 homologous neutralization (SOSIPs). We reasoned that the display of well-ordered trimers by high-density, particulate array would increase B cell activation compared to soluble trimers. Here, we present the design of liposomal nanoparticles displaying well-ordered Env spike trimers on their surface. Biophysical analysis, cryo-and negative stain electron microscopy, as well as binding analysis with a panel of broadly neutralizing antibodies confirm a high-density, well-ordered trimer particulate array. The Env-trimer-conjugated liposomes were superior to soluble trimers in activating B cells ex vivo and germinal center B cells in vivo. In addition, the trimer-conjugated liposomes elicited modest tier 2 homologous neutralizing antibodies. The trimer-conjugated liposomes represent a promising initial lead toward the development of more effective HIV vaccine immunogens.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available