Journal
CELL REPORTS
Volume 15, Issue 3, Pages 519-530Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2016.03.054
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Funding
- Cold Spring Harbor Laboratory NCI Cancer Center [CA455087]
- Alex's Lemonade Stand Foundation
- V Foundation
- Martin Sass Foundation
- Lauri Strauss Leukemia Foundation
- NIH [T32 GM008444, NCI RO1 CA174793]
- NCI [F30 CA186632]
- Burroughs-Wellcome Fund
- Leukemia & Lymphoma Society
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The bromodomain and extraterminal (BET) protein BRD4 can physically interact with the Mediator complex, but the relevance of this association to the therapeutic effects of BET inhibitors in cancer is unclear. Here, we show that BET inhibition causes a rapid release of Mediator from a subset of cis-regulatory elements in the genome of acute myeloid leukemia (AML) cells. These sites of Mediator eviction were highly correlated with transcriptional suppression of neighboring genes, which are enriched for targets of the transcription factor MYB and for functions related to leukemogenesis. A shRNA screen of Mediator in AML cells identified the MED12, MED13, MED23, and MED24 subunits as performing a similar regulatory function to BRD4 in this context, including a shared role in sustaining a block in myeloid maturation. These findings suggest that the interaction between BRD4 and Mediator has functional importance for gene-specific transcriptional activation and for AML maintenance.
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