4.4 Article

Molecular characterization & recombination analysis of complete enterovirus-88 isolated from acute flaccid paralysis cases in India

Journal

INDIAN JOURNAL OF MEDICAL RESEARCH
Volume 157, Issue 1, Pages 41-+

Publisher

WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/ijmr.IJMR_1767_19

Keywords

Acute flaccid paralysis; Bihar; enterovirus-88; India; next-generation sequencing; rash; recombinant; Uttar Pradesh

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The complete genome sequence of EV-B88 was identified and reported from Bihar and Uttar Pradesh in India using next-generation sequencing technique. The EV-B88 sequences retrieved in this study showed 83% similarity to the EV-B88 isolate from Bangladesh in 2001. Recombination events with echovirus-18 and echovirus-30 were observed in these EV-B88 isolates. This study increases the awareness about EV-B88 in India and highlights the need for further identification studies of other types of EV in India.
Background & objectives: Focus on non-polio enteroviruses (NPEVs) causing acute flaccid paralysis (AFP) due to myelitis has increased with the containment of the poliovirus. Enterovirus-B88 (EV-B88) has been associated with the AFP cases in Bangladesh, Ghana, South Africa, Thailand and India. In India, EV-B88 infection was linked to AFP a decade ago; however, to date, no complete genome has been made available. In this study, the complete genome sequence of EV-B88 was identified and reported from two different States (Bihar and Uttar Pradesh) in India using the next-generation sequencing technique. Methods: Virus isolation was performed on the three AFP suspected cases as per the WHO-recommended protocol. Samples showing cytopathic effects in the human Rhabdocarcinoma were labelled as NPEVs. Next-generation sequencing was performed on these NPEVs to identify the aetiological agent. The contiguous sequences (contigs) generated were identified, and reference-based mapping was performed. Results: EV-B88 sequences retrieved in our study were found to be 83 per cent similar to the EV-B88 isolate from Bangladesh in 2001 (strain: BAN01-10398; Accession number: AY843306.1). Recombination analyses of these samples demonstrate recombination events with sequences from echovirus-18 and echovirus-30. Interpretation & conclusions: Recombination events in the EV-B serotypes are known, and this work reconfirms the same for EV-B88 isolates also. This study is a step in increasing the awareness about EV-B88 in India and emphasizes future studies to be conducted in the identification of other types of EV present in India.

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