Facile one-step synthesis of gold nanoparticles using Viscum album and evaluation of their antibacterial potential

Nanostructure gold nanoparticles (Au NPs) are well-known biological active materials, synthesised under different environment-friendly approaches that has gained significant interest in the field of biomedicine. This study investigated a novel, fast, easy, cost-effective and the eco-friendly method to synthesise Au NPs from mediated Viscum album Linn plant extract, where the plant metabolites act as stabilising and reducing agents. The synthesised Au NPs were analysed by UV/Vis spectroscopy that gave strong signals and a sharp absorption peak at 545 nm due to the presence of surface plasmon resonance (SPR) bands. In addition, energy dispersive X-ray spectroscopy (EDX) showed that strong signals of Au NPs appeared at 9.7 and 2.3 keV, as the rays of light passed. X-ray diffraction recognised the crystalline material and provided information on the cell unit that the synthesised Au NPs are face-centreed cubic in structure. The diffraction of X-ray spectra showed intense peaks at 38.44 degrees, 44.7 degrees, 44.9 degrees and 77.8 degrees. The mediated V. album plant extracts and synthesised Au NPs were screened against gram-positive and gram-negative (Enterobacter, Salmonella typhi, Escheria coli and Bacillus subtilis) bacterial strains, confirming their antibacterial potential. Au NPs showed strong antibacterial activity due to its unique steric configuration. Au NPs damaged bacterial cell membrane leading to the leakage of the cytoplasm and death of the cell.

Automated summary

This study explored a novel, fast, easy, cost-effective, and eco-friendly method to synthesize nanostructure gold nanoparticles (Au NPs) using mediated Viscum album Linn plant extract. The synthesized Au NPs exhibited strong signals and a sharp absorption peak at 545 nm, indicating the presence of surface plasmon resonance bands. The antibacterial potential of the plant extracts and synthesized Au NPs was confirmed through screening against gram-positive and gram-negative bacterial strains.