Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 11, Issue 20, Pages 4523-4528Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d3tb00747b
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We designed and synthesized a new fluorescent probe, PerqdOH, which can monitor intracellular O-2(center dot-) during T. pallidum infection in real time. In vitro infection experiments showed decreased green fluorescence and enhanced red fluorescence simultaneously. The probe also exhibited a remarkable signal in treponemal lesions in a rabbit model. PerqdOH has great potential for application in clarifying the infectious process caused by T. pallidum in real time.
Syphilis, caused by Treponema pallidum (T. pallidum), is associated with the oxidative stress due to its inflammation-like symptom, and detecting the reactive oxygen species (ROS) is crucial for monitoring the infectious process. Herein, we design and synthesize a perylene-based tunable fluorescent probe, PerqdOH, which can detect endogenous O-2(center dot) during T. pallidum infection. The fluorescence peak shifted from 540 nm to 750 nm with increasing O-2(center dot-) levels. Besides, both decreased green fluorescence and enhanced red fluorescence could be observed simultaneously during the in vitro infection, providing the real-time monitoring of intracellular O-2(center dot-) caused by T. pallidum. Furthermore, the probe exhibited a remarkable signal in the treponemal lesions on the back of a rabbit model. Taken together, our synthesized PerqdOH holds great potential for application in clarifying the infectious process caused by T. pallidum in real time.
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