Severe Streptococcus equi Subspecies zooepidemicus Outbreak from Unpasteurized Dairy Product Consumption, Italy

Article Multidisciplinary Sciences

Comparison of MiSeq, MinION, and hybrid genome sequencing for analysis of Campylobacter jejuni

Jason M. Neal-McKinney et al.

Summary: The study compared Campylobacter genome assemblies generated from MiSeq and MinION data independently, as well as hybrid genome assemblies combining both data types. SPAdes assemblies had the most accurate nucleotide identity, while hybrid assemblies were the most contiguous. Assemblies generated only from MinION data using Canu were the least accurate. The hybrid sequencing approach was the most useful for detecting plasmids, large genome rearrangements, and repetitive elements.

SCIENTIFIC REPORTS (2021)

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Article Microbiology

Phylogenetic Analysis and Genome-Wide Association Study Applied to an Italian Listeria monocytogenes Outbreak

Alexandra Chiaverini et al.

Summary: The study investigated a severe outbreak of Listeria monocytogenes ST7 strain in Central Italy, linked to a meat food-producing plant contaminated by the outbreak strain, which reemerged in the same region causing another listeriosis case. The epidemic and reemergent strains were genetically closer to a ST7 clone with different PFGE profiles, and were associated with the pork production chain. The persistence and reemergence of the outbreak were likely due to both the presence of truly persistent clones and the repeated introduction of new ones, along with the contribution of prophage genes promoting their survival. Despite no significant genomic differences between the outbreak and reemergent strains, transcriptional factor and phage genes associated with the phage LP-114 helped differentiate the related clones detected during the outbreak.

FRONTIERS IN MICROBIOLOGY (2021)

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Letter Immunology