Immune activity of Ki-67 during nasal polyp development

OBJECTIVE: Nasal polyps are non-cancerous, soft painless growth of nasal mucosa. In this study, our aim was to investigate the Ki-67 expression level in nasal polyps by immunohistochemical method. PATIENTS AND METHODS: 30 patients with nasal polyps were included in this study. Nasal polyps were processed for paraffin wax embedding protocol. Samples were fixed and embedded in paraffin blocks. 5 mu m sections were stained with Hematoxylin-Eosin dye and immune stained with Ki-67 antibody. Sections were analyzed under light microscope. RESULTS: Blood parameters showed that white blood cells, hematocrit and platelet were higher than normal range. In sections of hematoxylin-eosin staining, elevated basal cells, thin basement membrane, leukocyte infiltration, collagen fibers degeneration were observed. Masson trichrome staining revealed that degenerative epithelial cells, detached basement membrane and edema were observed. Ki-67 expression was observed in mucosal epithelial cells, vascular endothelial cells and plasma cells in immune staining. CONCLUSIONS: Epithelial degeneration in nasal polyps and leukocyte infiltration induce nasal adenoma. Ki-67 expression may be a diagnostic tool for epithelial leukocyte formation.

Automated summary

The aim of this study was to investigate the expression level of Ki-67 in nasal polyps using immunohistochemical method. The results showed abnormal blood parameters and histological changes in nasal polyps, including cellular degeneration and leukocyte infiltration. Ki-67 expression was observed in mucosal epithelial cells, vascular endothelial cells and plasma cells. In conclusion, epithelial degeneration and leukocyte infiltration may contribute to the formation of nasal adenoma, and Ki-67 expression could be a diagnostic tool.