Journal
METALLOMICS
Volume 15, Issue 6, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/mtomcs/mfad030
Keywords
synchrotron X-ray fluorescence spectroscopy; correlative imaging; exogenous metal imaging; endogenous elemental imaging; metal-labelled antibodies; lanthanide X-ray fluorescence; synchrotron confocal X-ray fluorescence spectroscopy
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Exposure to exogenous particles is a growing concern for human health. To understand the associated biological response, it is important to characterize the concentrations, chemical species, distribution, and involvement of the stimulus with the tissue microanatomy. However, no single imaging technique can capture all these features at once, which limits correlative analyses. Developing synchronous imaging strategies that can identify multiple features simultaneously is crucial for assessing spatial relationships with greater confidence.
Exposure to exogenous particles is of increasing concern to human health. Characterizing the concentrations, chemical species, distribution, and involvement of the stimulus with the tissue microanatomy is essential in understanding the associated biological response. However, no single imaging technique can interrogate all these features at once, which confounds and limits correlative analyses. Developments of synchronous imaging strategies, allowing multiple features to be identified simultaneously, are essential to assess spatial relationships between these key features with greater confidence. Here, we present data to first highlight complications of correlative analysis between the tissue microanatomy and elemental composition associated with imaging serial tissue sections. This is achieved by assessing both the cellular and elemental distributions in three-dimensional space using optical microscopy on serial sections and confocal X-ray fluorescence spectroscopy on bulk samples, respectively. We propose a new imaging strategy using lanthanide-tagged antibodies with X-ray fluorescence spectroscopy. Using simulations, a series of lanthanide tags were identified as candidate labels for scenarios where tissue sections are imaged. The feasibility and value of the proposed approach are shown where an exposure of Ti was identified concurrently with CD45 positive cells at sub-cellular resolutions. Significant heterogeneity in the distribution of exogenous particles and cells can be present between immediately adjacent serial sections showing a clear need of synchronous imaging methods. The proposed approach enables elemental compositions to be correlated with the tissue microanatomy in a highly multiplexed and non-destructive manner at high spatial resolutions with the opportunity for subsequent guided analysis.
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