A DNA framework-based dual signal amplification biosensor for portable detection of SARS-CoV-2 and its mutations

We developed a rapid and accurate biosensor to detect SARS-CoV-2 and distinguish its mutations. Benefitting from a DNA framework-modified ordered interface and a dual signal amplification strategy, our biosensor could detect SARS-CoV-2 with a detection limit down to 10 fM. It performed well on pseudo virus and SARS-CoV-2 RNA standard materials, revealing the potential application in disease diagnosis and spread, in combination with a home-made smartphone.

Automated summary

We have developed a rapid and accurate biosensor capable of detecting SARS-CoV-2 and distinguishing its mutations. Utilizing a DNA framework-modified ordered interface and a dual signal amplification strategy, our biosensor has a detection limit as low as 10 fM. It has shown excellent performance on pseudo virus and SARS-CoV-2 RNA standard materials, indicating its potential application in disease diagnosis and spread when combined with a homemade smartphone.