Rhizobacteria isolated from xerophyte Haloxylon ammodendron manipulate root system architecture and enhance drought and salt tolerance in Arabidopsis thaliana

The objective of this study was to identify bacteria from the rhizosphere of the black saxaul (Haloxylon ammodendron) and test the possibility of using the bacteria for enhancement of drought and/or salt tolerance in the model plant, Arabidopsis thaliana. We collected rhizosphere and bulk soil samples from a natural habitat of H. ammodendron in Iran and identified 58 morphotypes of bacteria that were enriched in the rhizosphere. From this collection, we focused our further experiments on eight isolates. Microbiological analyses showed that these isolates have different levels of tolerance to heat, salt, and drought stresses, and showed different capabilities of auxin production and phosphorous solubilization. We first tested the effects of these bacteria on the salt tolerance of Arabidopsis on agar plate assays. The bacteria substantially influenced the root system architecture, but they were not effective in increasing salt tolerance significantly. Pot assays were then conducted to evaluate the effects of the bacteria on salt or drought tolerance of Arabidopsis on peat moss. Results showed that three of these bacteria (Pseudomonas spp. and Peribacillus sp.) effectively enhanced drought tolerance in Arabidopsis, so that while none of the mock-inoculated plants survived after 19 days of water withholding, the survival rate was 50-100% for the plants that were inoculated with these bacteria. The positive effects of the rhizobacteria on a phylogenetically-distant plant species imply that the desert rhizobacteria may be used to enhance abiotic stress in crops.

Automated summary

The objective of this study was to isolate bacteria from the rhizosphere of the black saxaul and test their potential for enhancing drought and/or salt tolerance in Arabidopsis thaliana. A total of 58 bacteria morphotypes were identified from rhizosphere samples, of which eight isolates were selected for further experiments. The bacteria showed varying levels of tolerance to heat, salt, and drought stresses, as well as different capabilities for auxin production and phosphorous solubilization. Three of the bacteria effectively enhanced drought tolerance in Arabidopsis, suggesting that desert rhizobacteria can be used to enhance abiotic stress in crops.