Efficacy of ozonated water as a PS in photodynamic therapy: A tool for dental caries management? An in vitro study

Background:The most prevalent noncommunicable disease in the world is dental caries; and when it is not adequately treated, it is usually associated with tooth loss or severe dental lesions. In fact, expensive care or tooth extraction may be necessary due to the negative effects dental caries have on general health. This is due to its frequent pain and secondary bacterial infections. The aim of this study was to investigate the activity of ozonated water as such and in combination with appropriate light radiation so as to perform a photodynamic treatment (PDT) against the cariogenic bacterium Streptococcus mutans. Design and methods:This work has been performed in vitro by using an S. mutans strain mainly structured in a biofilm status, reproducing the natural condition of the tooth infection. The ozone was tested at three different concentrations by using a commercial device able to generate different O-3 formulations in water. The PDT treatment requires an appropriate light wavelength, evaluated in this work through the UV-Vis adsorption spectrum of the ozonated water. Results:The obtained results suggested an effective and synergic property of O-3 and light at 460-470 nm against this microorganism. The most antibiofilm activity was observed using a concentration of ozone of 0.06 mg/L alone as well as with PDT treatment. Conclusions:The results are encouraging for additional research and in vitro/in vivo fresh experimental investigations to perform an exhaustive antimicrobial treatment protocol against the S. mutans tooth infection.

Automated summary

The aim of this study was to investigate the activity of ozonated water alone and in combination with appropriate light radiation in photodynamic treatment (PDT) against the cariogenic bacterium Streptococcus mutans. The UV-Vis absorption spectrum of the ozonated water was evaluated to determine the appropriate light wavelength. The results showed that a concentration of ozone of 0.06 mg/L had the most effective antibiofilm activity against this microorganism when used alone or with PDT treatment. These findings provide encouraging evidence for further research and in vitro/in vivo experimental investigations for a comprehensive antimicrobial treatment protocol against S. mutans tooth infection.