Sequencing for oxidative DNA damage at single-nucleotide resolution with click-code-seq v2.0

Oxidative damage to DNA nucleotides has many cellular outcomes that could be aided by the development of sequencing methods. Herein, the previously reported click-code-seq method for sequencing a single damage type is redeveloped to enable the sequencing of many damage types by making simple changes to the protocol (i.e., click-code-seq v2.0).

Automated summary

The previously reported click-code-seq method has been redesigned to enable the sequencing of many damage types by making simple changes to the protocol (i.e., click-code-seq v2.0), which could aid in understanding the cellular outcomes of oxidative damage to DNA nucleotides.