Backscatter from therapeutic doses of ionizing irradiation does not impair cell migration on titanium implants in vitro

ObjectiveThe influence of radiation backscatter from titanium on DNA damage and migration capacity of human osteoblasts (OBs) and mesenchymal stem cells (MSCs) may be critical for the osseointegration of dental implants placed prior to radiotherapy. In order to evaluate effects of radiation backscatter, the immediate DNA damage and migration capacity of OBs and MSCs cultured on titanium or plastic were compared after exposure to ionizing irradiation. Materials and methodsHuman OBs and MSCs were seeded on machined titanium, moderately rough fluoride-modified titanium, or tissue culture polystyrene, and irradiated with nominal doses of 2, 6, 10, or 14 Gy. Comet assay was performed immediately after irradiation, while a scratch wound healing assay was initiated 24 h post-irradiation. Fluorescent live cell imaging documented the migration.ResultsDNA damage increased with higher dose and with backscatter from titanium, and MSCs were significantly more affected than OBs. All doses of radiation accelerated the cell migration on plastic, while only the highest dose of 10 Gy inhibited the migration of both cell types on titanium.ConclusionsHigh doses (10 Gy) of radiation inhibited the migration capacity of both cell types on titanium, whereas lower doses (2 and 6 Gy) did not affect the migration of either OBs or MSCs.

Automated summary

The influence of radiation backscatter from titanium on DNA damage and migration capacity of human osteoblasts and mesenchymal stem cells was investigated. Higher doses of radiation and backscatter from titanium increased DNA damage, with mesenchymal stem cells being more affected. High doses of radiation inhibited cell migration on titanium, while low doses did not affect migration capacity.