Reversible Increase in Resistance of A-431 Carcinoma Cells to TRAIL-Induced Apoptosis in Confluent Cultures Corresponds to a Decrease in Expression of DR4 and DR5 Receptors

Article Biochemistry & Molecular Biology

Macrophage-like THP-1 Cells Derived from High-Density Cell Culture Are Resistant to TRAIL-Induced Cell Death via Down-Regulation of Death-Receptors DR4 and DR5

Yana Vladimirovna Lomovskaya et al.

Summary: The resistance of leukemic cells to TRAIL-induced apoptosis is a major challenge in the treatment of leukemia. In this study, researchers discovered that human acute myeloid leukemia cells, when subjected to high-density culture conditions, displayed a macrophage-like phenotype and increased resistance to TRAIL-induced cell death. This resistance can be attributed to a decrease in the expression of death receptors DR4 and DR5 on the leukemic cells. The findings suggest that stress conditions in high-density cell cultures may contribute to tumor progression by promoting TRAIL resistance.

BIOMOLECULES (2022)

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Article Biochemistry & Molecular Biology

The Increase in the Drug Resistance of Acute Myeloid Leukemia THP-1 Cells in High-Density Cell Culture Is Associated with Inflammatory-like Activation and Anti-Apoptotic Bcl-2 Proteins

Margarita Kobyakova et al.

Summary: It is known that cell culture density can modulate the drug resistance of acute myeloid leukemia (AML) cells. In this study, the drug sensitivity of AML cells in high-density cell cultures was investigated. The results showed that AML cells in high-density cell cultures were significantly more resistant to DNA-damaging drugs and recombinant ligand izTRAIL compared to those in low-density cell cultures. Further analysis revealed that the increased drug resistance of AML cells in high-density cultures was associated with the accumulation of inflammatory cytokines and the activation of NF-kB-dependent inflammatory-like cells.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

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Article Multidisciplinary Sciences

Overcoming TRAIL-resistance by sensitizing prostate cancer 3D spheroids with taxanes

Korie A. Grayson et al.

Summary: Three-dimensional spheroid cultures better mimic cell-cell and matrix interactions in solid tumors than traditional 2D cultures, but maintaining uniform spheroid size remains a challenge. Pretreatment with CBZ or DTX enhances TRAIL-induced apoptosis in DU145 cell cultures, while PC3 spheroids exhibit stronger resistance to combination therapy.

PLOS ONE (2021)

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Article Biochemistry & Molecular Biology