Journal
ADVANCED HEALTHCARE MATERIALS
Volume 5, Issue 23, Pages 3046-3055Publisher
WILEY-BLACKWELL
DOI: 10.1002/adhm.201600699
Keywords
biofunctionalization; electrospinning; endothelialization; pericytes; tissue engineered vascular graft
Funding
- Medical Research Council
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council UK Regenerative Medicine Platform Hub Acellular Approaches for Therapeutic Delivery [MR/K026682/1]
- ERC [616417]
- Medical Research Council (MRC) [MR/J015350/1]
- Heart Research UK
- BHF Centre of Regenerative Medicine [RM/13/2/30158]
- Fellowship from the Swiss National Science Foundation SNF [P2BEP3_152091, P300PB_161072]
- MRC [MR/K026682/1, MR/J015350/1, G0900912, MR/N027086/1] Funding Source: UKRI
- British Heart Foundation [PG/10/81/28606, FS/16/9/32012, SP/12/7/29572] Funding Source: researchfish
- Medical Research Council [G0900912, MR/N027086/1, MR/J015350/1, MR/K026682/1] Funding Source: researchfish
- Swiss National Science Foundation (SNF) [P300PB_161072, P2BEP3_152091] Funding Source: Swiss National Science Foundation (SNF)
Ask authors/readers for more resources
The development of synthetic vascular grafts for coronary artery bypass is challenged by insufficient endothelialization, which increases the risk of thrombosis, and the lack of native cellular constituents, which favors pathological remodeling. Here, a bifunctional electrospun poly(epsilon-caprolactone) (PCL) scaffold with potential for synthetic vascular graft applications is presented. This scaffold incorporates two tethered peptides: the osteopontin-derived peptide (Adh) on the luminal side and a heparin-binding peptide (Hep) on the abluminal side. Additionally, the abluminal side of the scaffold is seeded with saphenous vein-derived pericytes (SVPs) as a source of proangiogenic growth factors. The Adh peptide significantly increases endothelial cell adhesion, while the Hep peptide promotes accumulation of vascular endothelial growth factor secreted by SVPs. SVPs increase endothelial migration both in a transwell assay and a modified scratch assay performed on the PCL scaffold. Seeding of SVPs on the abluminal/Hep side of the scaffold further increases endothelial cell density, indicating a combinatory effect of the peptides and pericytes. Finally, SVP-seeded scaffolds are preserved by freezing in a xeno-free medium, maintaining good cell viability and function. In conclusion, this engineered scaffold combines patient-derived pericytes and spatially organized functionalities, which synergistically increase endothelial cell density and growth factor retention.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available