Journal
ACS SYNTHETIC BIOLOGY
Volume 5, Issue 12, Pages 1466-1474Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.6b00085
Keywords
surface display; silica binding peptides; autocementation; Yarrowia lipolytica
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Funding
- National Education Base of Bioscience Research from the National Science Foundation of China [NSFC-J1103514]
- Teaching Research Program of HUST [2016047, 2014001]
- Teaching Research Program of Hubei Province [2014070]
- Qiming College grant for innovation from HUST
- National Undergraduate Innovation grant from the College of Life Science and Technology of HUST
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With the development of civil engineering, the demand for suitable cementation materials is increasing rapidly. However, traditional cementation methods are not eco-friendly enough and more sustainable approach such as biobased cementation is required. To meet such demand, Euk.cement, a living eukaryotic cell-based biological autocementation kit, was created in this work. Through the surface display of different silica binding peptides on the fungus Yarrowia lipolytica, Euk.cement cells can immobilize onto any particles with a silica containing surface with variable binding intensity. Meanwhile, recombinant MCFP3 released from the cells will slowly consolidate this binding of cells to particles. The metabolism of immobilized living cells will finally complete the carbonate sedimentation and tightly stick the particles together. The system is designed to be initiated by blue light, making it controllable. This autocementation kit can be utilized for industrial and environmental applications that fit our concerns on making the cementation process eco-friendly.
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