Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain

When a monkeypox virus outbreak began in several parts of the world in May 2022, timely and accurate diag-nosis became mandatory. In our laboratory, a real-time quantitative PCR was designed and evaluated in several patient samples and compared with isolation results. Ge-nomic viral load was related to virus viability.

Automated summary

When a monkeypox virus outbreak occurred globally in May 2022, it became crucial to have timely and accurate diagnosis. In our laboratory, we developed and assessed a real-time quantitative PCR method, which was compared with isolation results. The genomic viral load was found to be associated with virus viability.