Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spread

Duck plague virus (DPV) is one of the major infectious and fatal diseases of geese, ducks, and other wild waterfowl. The DPV UL49 gene product VP22 is one of the most abundant tegument proteins. However, the role of the DPV VP22 is enigmatic to be clarified. In this study, we found deletion of the UL49 gene resulted in reduced viral growth curve and smaller plaque size in duck embryo fibroblast (DEF) cells, confirming that DPV VP22 is required for efficient viral growth in vitro. In addition, deletion of the UL49 gene inhibited the secondary envelopment of the virus, the release of viral particles, and the spread of viruses between cells. Our study signified the importance of VP22 for DPV secondary envelopment, release, cell-to-cell spread, and accumulation of viral RNA. These findings provide a basis for further study of the function of VP22 in DPV or other herpesviruses.

Automated summary

Duck plague virus (DPV) is a significant infectious and fatal disease for geese, ducks, and other wild waterfowl. Our study found that the VP22 protein in DPV plays a crucial role in viral growth, spread, and RNA accumulation, providing a foundation for further research on the function of VP22 in DPV or other herpesviruses.