Combined omics approaches expose metabolite-microbiota correlations in grape berries of three cultivars of Douro wine region

This study hypothesized the existence of cultivar-associated correlations between grape berry metabolites and its microbial residents, in Douro wine region. Integrated metabolomics with metabarcoding showed that the microbial biodiversity is not associated to berry sugar concentration, but closely connected to the profile of amino acids, flavonoids and wax compounds, which drove cultivar differentiation together with the prevalence of pathogenic fungi, yeasts and bacteria, mainly Dothideomycetes and Gammaproteobacteria. Over 7000 metabolite-microbiota correlations with & rho; >|0.99| exposed a core of 15 metabolites linked to 11 microbial taxa. Serine, oxalate, cyanidin-3-O-glucoside, petunidin-3-O-glucoside, gallic acid, germanicol, sitosterol and erythrodiol correlated negatively to the abundance of most taxa, including Alternaria, Aureobasidium, Pseudopithomyces, Pseudomonas and Sphingomonas. In contrast, phenylalanine, asparagine, alanine, (epi)gallocatechin and procyanidin gallate mediated positive metabolite-OTU correlations. E. necator and A. carbonarius correlated negatively with stigmasterol and amyrin. Complex fungi-bacteria relationships ruled by Dothideomycetes and Alphaproteobacteria further suggest tight host-microbe interactions at the carposphere.

Automated summary

This study investigates the correlation between grape berry metabolites and its microbial residents in the Douro wine region. The results show that microbial biodiversity is closely related to the profile of amino acids, flavonoids, and wax compounds, which contribute to cultivar differentiation and the presence of pathogenic fungi, yeasts, and bacteria. Over 7000 metabolite-microbiota correlations were found, with a core of 15 metabolites linked to 11 microbial taxa. Certain metabolites correlated negatively or positively with the abundance of specific microbial taxa, indicating a complex host-microbe interaction at the berry surface.