4.3 Article

Single-Cell RNA-seq Analysis of a Human Embryonic Stem Cell to Endothelial Cell System Based on Transcription Factor Overexpression

Related references

Note: Only part of the references are listed.
Article Anatomy & Morphology

Tal2 is required for generation of GABAergic neurons in the zebrafish midbrain

Ruihong Wang et al.

Summary: The expression of multiple transcription factors, including tal2, gata2a, and gata3, is associated with the generation of GABAergic neurons in the zebrafish midbrain. Among them, tal2 is crucial for the neurogenesis of GABAergic neurons in the nucleus of the medial longitudinal fasciculus (nMLF), while the function of gata2a and gata3 is dispensable.

DEVELOPMENTAL DYNAMICS (2023)

Article Hematology

Endothelial MEKK3-KLF2/4 signaling integrates inflammatory and hemodynamic signals during definitive hematopoiesis

Yiqing Yang et al.

Summary: This study identifies the MEKK3-KLF2/4 signaling pathway as essential for the specification of hemogenic endothelial cells and their transition to intraaortic hematopoietic cluster cells during embryonic hematopoiesis. Inflammatory mediators and fluid shear forces can activate this signaling pathway and promote the process.

BLOOD (2022)

Article Cell & Tissue Engineering

Anniversary reflections: Inspiring discoveries and the future of the field

Madeline Lancaster et al.

Cell Stem Cell (2022)

Article Biotechnology & Applied Microbiology

Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells

Diego Balboa et al.

Summary: Transplanting pancreatic islet cells derived from human pluripotent stem cells has great potential for treating diabetes. This study generated functionally mature stem-cell-derived islets (SC-islets) using an optimized protocol and compared them comprehensively to primary adult islets. The results showed that SC-islets exhibited similar functional properties to adult islets in terms of insulin secretion, electrophysiology, signaling, and exocytosis. Additionally, single-cell transcriptomics revealed a continuous maturation trajectory of SC-islets in vitro and during engraftment in mice, closely resembling that of primary islets.

NATURE BIOTECHNOLOGY (2022)

Review Biochemistry & Molecular Biology

Regenerative and restorative medicine for eye disease

Russell N. Van Gelder et al.

Summary: This review discusses the advancement in cell-based therapy, gene therapy, and prosthetics for visual function restoration in degenerative retinal disease, as well as the current status of human clinical trials and preclinical studies.

NATURE MEDICINE (2022)

Article Cell & Tissue Engineering

Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Human Developmental Hematopoiesis

Anna Maria Ranzoni et al.

Summary: Using single-cell RNA sequencing and single-cell assay for transposase-accessible chromatin sequencing, researchers identified three highly proliferative oligopotent progenitor populations downstream of hematopoietic stem cells (HSCs)/multipotent progenitors (MPPs) in human fetal liver and bone marrow. They observed dynamic changes in chromatin accessibility and lineage-specific transcription factor activity along the differentiation trajectory, providing a useful framework for future investigation of human developmental hematopoiesis.

CELL STEM CELL (2021)

Article Cardiac & Cardiovascular Systems

Mapping the Endothelial Cell S-Sulfhydrome Highlights the Crucial Role of Integrin Sulfhydration in Vascular Function

Sofia-Iris Bibli et al.

Summary: This study investigated the S-sulfhydrome in human endothelial cells and found that S-sulfhydration plays a crucial role in regulating endothelial cell functions, especially in integrins and mechanotransduction. Impaired S-sulfhydration was associated with vascular dysfunction, while H2Sn supplementation improved vascular reactivity, suggesting a potential therapeutic target for vascular diseases.

CIRCULATION (2021)

Article Hematology

Disruption of a GATA2-TAL1-ERG regulatory circuit promotes erythroid transition in healthy and leukemic stem cells

Julie A. I. Thoms et al.

Summary: This study reveals the presence of the fully connected heptad circuit in both healthy hematopoietic stem and progenitor cells (HSPCs) and leukemic cells, with only minor alterations in acute myeloid leukemia (AML). The subcircuit consisting of GATA2, TALI, and ERG regulates stem cell-to-erythroid transition in both healthy and leukemic cells, suggesting the potential for manipulating these factors to facilitate specific cell-type transitions and overcome dysregulated hematopoiesis.

BLOOD (2021)

Article Multidisciplinary Sciences

3D genome alterations associated with dysregulated HOXA13 expression in high-risk T-lineage acute lymphoblastic leukemia

Lu Yang et al.

Summary: Integrated analyses of 3D genome alterations and differential gene expressions in T-ALL patients revealed the potential regulatory role of translocation-mediated neo-loops on key transcription factors, such as HOXA13, associated with poor prognosis. The study highlights the importance of 3D genome alterations in the etiology and prognosis of T-ALL.

NATURE COMMUNICATIONS (2021)

Article Cell & Tissue Engineering

Oct4-dependent FoxC1 activation improves the survival and neovascularization of mesenchymal stem cells under myocardial ischemia

Zhou Ji et al.

Summary: The study found that upregulation of FoxC1 in cardiac endothelial cells after ischemic injury promotes intrinsic angiogenesis. Inducing a vascular microenvironment by overexpressing FoxC1 enhances survival and neovascularization of MSCs under hypoxic conditions, potentially improving cardiac repair.

STEM CELL RESEARCH & THERAPY (2021)

Article Multidisciplinary Sciences

A hepatocyte differentiation model reveals two subtypes of liver cancer with different oncofetal properties and therapeutic targets

Ming Liu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Review Cell & Tissue Engineering

Pluripotent Stem Cell-Based Cell Therapy- Promise and Challenges

Shinya Yamanaka

CELL STEM CELL (2020)

Review Genetics & Heredity

RNA sequencing: the teenage years

Rory Stark et al.

NATURE REVIEWS GENETICS (2019)

Review Biochemistry & Molecular Biology

The Human Transcription Factors

Samuel A. Lambert et al.

Review Hematology

Transcription factor defects causing platelet disorders

Martina E. Daly

BLOOD REVIEWS (2017)

Article Engineering, Biomedical

Human Skin Constructs with Spatially Controlled Vasculature Using Primary and iPSC-Derived Endothelial Cells

Hasan E. Abaci et al.

ADVANCED HEALTHCARE MATERIALS (2016)

Article Biotechnology & Applied Microbiology

Pooling across cells to normalize single-cell RNA sequencing data with many zero counts

Aaron T. L. Lun et al.

GENOME BIOLOGY (2016)

Review Hematology

Haematopoietic and immune defects associated with GATA2 mutation

Matthew Collin et al.

BRITISH JOURNAL OF HAEMATOLOGY (2015)

Article Biochemistry & Molecular Biology

Tbx1 regulates brain vascularization

Sara Cioffi et al.

HUMAN MOLECULAR GENETICS (2014)

Article Cell Biology

TGF-β Signaling Mediates Endothelial-to-Mesenchymal Transition (EndMT) During Vein Graft Remodeling

Brian C. Cooley et al.

SCIENCE TRANSLATIONAL MEDICINE (2014)

Review Genetics & Heredity

Transcription factors: from enhancer binding to developmental control

Francois Spitz et al.

NATURE REVIEWS GENETICS (2012)

Article Hematology

Embryonic Stem Cell-Derived Endothelial Cells Engraft Into the Ischemic Hindlimb and Restore Perfusion

Ngan F. Huang et al.

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY (2010)

Article Biochemical Research Methods

Fast and SNP-tolerant detection of complex variants and splicing in short reads

Thomas D. Wu et al.

BIOINFORMATICS (2010)

Editorial Material Cell Biology

Journal club FROM MYOD1 TO IPS CELLS

Konrad Hochedlinger

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2010)

Article Multidisciplinary Sciences

In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state

Marius Wernig et al.

NATURE (2007)

Article Developmental Biology

The T cell oncogene Tal2 is necessary for normal development of the mouse brain

K Bucher et al.

DEVELOPMENTAL BIOLOGY (2000)