Journal
THERANOSTICS
Volume 6, Issue 11, Pages 2000-2014Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.15102
Keywords
DC vaccine; immunotherapy; antigen delivery; DC migration tracking; magnetic targeting
Categories
Funding
- National Natural Science Foundation of China [91442201]
- China Postdoctoral Science Foundation [2013T60721]
- State Key Laboratory of Bioelectronics of Southeast University
- Fundamental Research Funds for the Central Universities [HUST: 2015ZDTD014]
- WNLO
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Dendritic cell (DC) migration to the lymph node is a key component of DC-based immunotherapy. However, the DC homing rate to the lymphoid tissues is poor, thus hindering the DC-mediated activation of antigen-specific T cells. Here, we developed a system using fluorescent magnetic nanoparticles (alpha-AP-fmNPs; loaded with antigen peptide, iron oxide nanoparticles, and indocyanine green) in combination with magnetic pull force (MPF) to successfully manipulate DC migration in vitro and in vivo. alpha-AP-fmNPs endowed DCs with MPF-responsiveness, antigen presentation, and simultaneous optical and magnetic resonance imaging detectability. We showed for the first time that alpha-AP-fmNP-loaded DCs were sensitive to MPF, and their migration efficiency could be dramatically improved both in vitro and in vivo through MPF treatment. Due to the enhanced migration of DCs, MPF treatment significantly augmented antitumor efficacy of the nanoparticle-loaded DCs. Therefore, we have developed a biocompatible approach with which to improve the homing efficiency of DCs and subsequent anti-tumor efficacy, and track their migration by multi-modality imaging, with great potential applications for DC-based cancer immunotherapy.
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