Journal
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 30, Issue 36, Pages 86484-86497Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-023-28635-1
Keywords
Fusarium verticillioides; Enzyme; Submerged fermentation; Optimization; Malathion; GC; MS
Categories
Ask authors/readers for more resources
In this study, a cutinolytic fungus was isolated from the soil and identified as belonging to the genus Fusarium, specifically Fusarium verticillioides, based on 18s rDNA sequencing. The composition of the medium for enhanced cutinase production was optimized using classical and statistical methods. The optimized conditions resulted in a 14.57-fold increase in enzyme activity, with a maximum cutinase activity of 626.6 IUml(-1).
Cutinase, a multifunctional enzyme, has shown great potential in environmental applications such as degradation of plastics and some commonly used insecticides. To overcome these environmental threatening problems, attempts should be made to enhance enzyme production. In the present study, a cutinolytic fungus was isolated from the soil. Based on 18 s rDNA sequencing, it was found that isolate AR08 belongs to the genus Fusarium and clades with Fusarium verticillioides. Optimization of medium composition for enhancement in cutinase production was done using. classical and statistical methods. Firstly, key factors were selected by one variable at a time (OVAT) method, then by Plackett- Burman design. Concentration of these important factors was optimized by Central Composite design. A total of 30 experiments were conducted and the optimized concentration of sodium nitrate, dipotassium hydrogen phosphate, flaxseed oil and zinc sulphate were found to be 0.455%, 0.305%, 2% and 0.0355% respectively. The result of ANOVA (analysis of variance) test revealed that p value was significant for the model. Interaction between flaxseed oil and sodium nitrate was found to have a positive effect on cutinase production. A 14.57 fold increase in enzyme activity was found under optimized conditions with the maximum cutinase activity of 626.6 IUml(-1).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available