Detecting diagnostic features in MS/MS spectra of post-translationally modified peptides

Post-translational modifications are an area of great interest in mass spectrometry-based proteomics, with a surge in methods to detect them in recent years. However, post-translational modifications can introduce complexity into proteomics searches by fragmenting in unexpected ways, ultimately hindering the detection of modified peptides. To address these deficiencies, we present a fully automated method to find diagnostic spectral features for any modification. The features can be incorporated into proteomics search engines to improve modified peptide recovery and localization. We show the utility of this approach by interrogating fragmentation patterns for a cysteine-reactive chemoproteomic probe, RNA-crosslinked peptides, sialic acid-containing glycopeptides, and ADP-ribosylated peptides. We also analyze the interactions between a diagnostic ion's intensity and its statistical properties. This method has been incorporated into the open-search annotation tool PTM-Shepherd and the FragPipe computational platform. Protein modifications increase the complexity of data analysis in mass spectrometry-based proteomics, which may impair the comprehensive mapping of modification sites. Here, the authors develop an algorithm to extract diagnostic fragmentation patterns to improve modified peptide recovery and localization.

Automated summary

Post-translational modifications are important in proteomics, but can complicate searches for modified peptides. The authors present an automated method to find diagnostic spectral features for any modification, improving peptide recovery and localization. They demonstrate the utility of this approach for various modifications and analyze the interactions between ion intensity and statistical properties. This method has been incorporated into PTM-Shepherd and FragPipe.