Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep24484
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Funding
- X-ray Free-Electron Laser Priority Strategy Program (MEXT)
- Japan Society for the Promotion of Science (KAKENHI) [25650026]
- Research Acceleration Program of the Japan Science and Technology Agency
- Platform for Drug Discovery, Informatics, and Structural Life Science (MEXT)
- NRF through SRC [NRF-2015R1A5A1009962]
- Grants-in-Aid for Scientific Research [25650026] Funding Source: KAKEN
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The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-angstrom resolution.
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