Analysis of the antimelanogenic activity of zinc and selenium in vitro

Melasma is an acquired chronic condition characterized by hyperchromic patches in photo-exposed areas. The search for new compounds for the treatment of melasma without side effects is constant. In this context, the aim of this study was to investigate the in vitro cytotoxic and antimelanogenic effects of the trace elements Zinc (Zn) and Selenium (Se). In this study, we evaluated the effects of 30 & mu;M hydroquinone, this concentration did not alter mitochondrial function (MTT assay), but increased the percentage of necrotic cells and levels of reactive species. Furthermore, it showed no influence on tyrosinase activity and melanin content. Unlike hydroquinone, exposure for 48 h to 100 & mu;M Zn and 1 and 5 & mu;M Se had no significant influence on the analysis of reactive species, as well as on the percentage of necrotic cells. Still, specifically in relation to 100 & mu;M Zn, it decreased the melanin content. Given the above, the trace elements Zn and Se did not show toxicity at the concentrations tested and Zn showed a promising effect, however, the mechanism needs to be better explored in order to contribute to new and updated research in the fight against melasma with a perspective of therapeutic use.

Automated summary

The study aimed to investigate the cytotoxic and antimelanogenic effects of trace elements Zinc (Zn) and Selenium (Se) in vitro. The results showed that 100 μM Zn had no toxic effects on cells and could reduce melanin content. However, further research is needed to determine the mechanism of action for potential therapeutic use in the treatment of melasma.