Viscosity probes towards different organelles with red emission based on an identical hemicyanine structure

A series of viscosity probes targeting different organelles were obtained using a single hemicyanine dye as the matrix structure. Specifically, probes 1a-d were obtained by introducing four amines (6-amino-2H-chromen-2-one, N-(2-aminoethyl)-4-methylbenzenesulfonamide, dodecan-1-amine and N,N diphenylbenzene-1,4-diamine) into the indole hemicyanine dye of the carboxylic acid with a D-p-A structure. Their maximum absorption wavelengths were in the range 570-586 nm and they had relatively large molar absorption coefficients, while their maximum emission wavelengths in the red light region were in the range 596-611 nm. Moreover, their fluorescence intensity in glycerol was 35-184 times higher than that in phosphate buffer solution (PBS). The lg(Fl) and lg ? of probes 1a-d showed good linearity with high correlation coefficients according to the Forster-Hoffman equation. In addition, cell staining experiments demonstrated that 1a-c could target lysosomes, endoplasmic reticulum and mitochondria, respectively. They could also undergo viscosity-detectable changes in the corresponding organelles under the action of the corresponding ion carriers.

Automated summary

A series of viscosity probes targeting different organelles were obtained using a single hemicyanine dye as the matrix structure. These probes had relatively large molar absorption coefficients in the red light region and their fluorescence intensity in glycerol was 35-184 times higher than that in phosphate buffer solution (PBS). Cell staining experiments demonstrated that these probes could target lysosomes, endoplasmic reticulum and mitochondria, and undergo viscosity-detectable changes in the corresponding organelles.