An electrochemical approach for the prediction of & UDelta;(9)-tetrahydrocannabinolic acid and total cannabinoid content in Cannabis sativa L.

Two electrochemical sensors are proposed here for the first time for the fast screening of cannabinoids in Cannabis sativa L. plant material (inflorescences). The accurate control of cannabinoid content is important for discriminating between recreational, i.e. illegal, and fibre-type C. sativa samples, which differ mainly according to the amount of & UDelta;(9)-tetrahydrocannabinol (& UDelta;(9)-THC) and & UDelta;(9)-tetrahydrocannabinolic acid (& UDelta;(9)-THCA). Two screen printed electrodes obtained using different electrode materials were tested for the analysis of extracts from recreational and fibre-type C. sativa and their performance was compared with a consolidated method based on high-performance liquid chromatography (HPLC). The voltammetric responses recorded in the different samples reflected the compositional differences of the recreational and fibre-type extracts in accordance with the results of HPLC analyses. Moreover, the quantification of & UDelta;(9)-THCA and the total cannabinoid content on the basis of the intensity of the peaks of the voltammograms was possible through a simple and fast electrochemical procedure.

Automated summary

Two electrochemical sensors were developed for rapid screening of cannabinoids in Cannabis sativa L. plant material. Control of cannabinoid content is important for distinguishing between recreational and fibre-type C. sativa samples, which differ in their THCA and THC levels.