Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep28107
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Funding
- Austrian Academy of Sciences
- University of Zurich (Forschungskredit)
- Swiss National Science Foundation [PP00P2_157607]
- DOC fellowship from the Austrian Academy of Sciences
- Swiss National Science Foundation (SNF) [PP00P2_157607] Funding Source: Swiss National Science Foundation (SNF)
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Mass spectrometry-based in vitro kinase screens play an essential role in the discovery of kinase substrates, however, many suffer from biological and technical noise or necessitate genetically-altered enzyme-cofactor systems. We describe a method that combines stable gamma-[O-18(2)]-ATP with classical in vitro kinase assays within a contemporary quantitative proteomic workflow. Our approach improved detection of known substrates of the non-receptor tyrosine kinase ABL1; and identified potential, new in vitro substrates.
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