A colorimetric and fluorometric dual-mode probe for Cu(2+)detection based on functionalized silver nanoparticles

A novel colorimetric/fluorescent probe (AgNPs-GSH-Rh6G2) was prepared by linking silver nanoparticles (AgNPs) with rhodamine 6G derivative (Rh6G2) using glutathione (GSH) as a linker molecule. The prepared probe showed obvious fluorescence change and colorimetric response after adding copper ions. Based on this phenomenon, a colorimetric/fluorescence dual-mode detection method was constructed to recognize copper ions. The linear ranges of fluorescence detection and colorimetric detection were 0.10 to 0.45 mM and 0.15 to 0.65 mM, respectively, and the limit of detection were 0.18 & mu;M and 24.90 & mu;M. In addition, the dual-mode probe has achieved satisfactory results in the detection of copper ions in sediment samples. The successful construction of AgNPs-GSH-Rh6G2 not only provide a reliable tool for the detection of copper ions, but also shed light on a new idea for the multi-mode development of the detection platform.

Automated summary

A novel colorimetric/fluorescent probe (AgNPs-GSH-Rh6G2) was prepared by linking silver nanoparticles (AgNPs) with rhodamine 6G derivative (Rh6G2) using glutathione (GSH) as a linker molecule. The probe exhibited significant fluorescence change and colorimetric response upon addition of copper ions. A colorimetric/fluorescence dual-mode detection method was developed based on this phenomenon to selectively detect copper ions. The probe showed good sensitivity with linear ranges of 0.10-0.45 mM for fluorescence detection and 0.15-0.65 mM for colorimetric detection, and the detection limits were 0.18 μM and 24.90 μM, respectively. The dual-mode probe also demonstrated satisfactory results in detecting copper ions in sediment samples.